Evidence From Humans
 
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Immunoperoxidase detection of polycyclic aromatic hydrocarbon-DNA adducts in breast tissue sections
Motykiewicz, G., Malusecka, E., Michalska, J., Kalinowska, E., Wloch, J., Butkiewicz, D., Mazurek, A., Lange, D., Perera, F. P., Santella, R. M. Cancer Detect Prev. 2001. 25:4, 328-35.
Topic area
Environmental pollutant - PAH
Study design
Case-control
Study Participants
Menopausal Status
The menopausal status of women included in this study is listed here.
Pre menopausal
Number of Controls
Controls: 30
Participant selection: Inclusion and exclusion criteria
Criteria used to select participants in the study.
Residents of Silesia aged 31-72 who were operated on in 1995 at a surgery clinic. Controls were women with benign breast disease.
Comment about participation selection
51% of participants were younger than 50, young for breast cancer. Small number of participants. Benign breast disease patients are not representative of a general population.
Exposure Investigated
Exposures investigated
PAH from air pollution, smoking measured in tissue by adducts detected in paraffin sections by polyclonal antiserum-based immunoperoxidase method, GSTM1 and CYP1A1 exon 7 Ile/Val genotypes
How exposure was measured
Questionnaire, in person Biological
Exposure assessment comment
Interpretation is not well understood. For example, the relevant exposure time period influencing adduct levels is unknown.
Ethnic groups with separate analysis
If this study provided a separate analysis by ethnic or racial group, the groups are listed here.
No
Genetic characterization included
If the study analyzed relationships between environmental factors and inherited genetic variations, this field will be marked “Yes.” “No”, if not.
Yes
Description of major analysis
Nonparametric Mann-Whitney U test, multiple regression
Strength of associations reported
Higher intensity of staining among benign breast disease patients (p < .001). No significant differences between smokers and non-smokers. No significant effects of genetic polymorphisms. No significant correlation between adduct levels and age of donor. Staining was associated with higher weight (p -.03).
Results Comments
Small numbers of study participants. Results do not clarify the significance of PAH-DNA adducts as an exposure measure.
Author address
Division of Environmental Health Sciences, Joseph L. Mailman School of Public Health, Columbia University, Gliwice, Poland.
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