Environment and Breast Cancer: Science Review
AboutCancer studies: Experimental details
Huff JE, Melnick RL, Solleveld HA, Haseman JK, Powers M, Miller RA. Multiple organ carcinogenicity of 1,3-butadiene in B6C3F1 mice after 60 weeks of inhalation exposure. Science 1985;227(4686):548-9
Notes
Increased malignant mammary tumors in female mice. 0, 625, 1250 ppm, 6 hrs/d, 5 d/wk for 60-61 wks. 0/50, 2/49, 6/49 carcinoma
Increased malignant mammary tumors in female mice. 0, 625, 1250 ppm, 6 hrs/d, 5 d/wk for 60-61 wks. 0/50, 2/49, 6/49 carcinoma
Route
Route of chemical administration: dermal, inhalation, gavage (delivery directly into the
stomach), in feed, subcutaneous injection (under the skin), or intraperitoneal injection (into the
cavity that contains the abdominal organs).
inhalation
Doses
Dosage, frequency, and duration of treatment; the sizes of the groups of animals
involved and what age the animals were at the beginning of the study.
0, 625, 1250 ppm, 6 hrs/d, 5 d/wk for 60-61 wks
50 animals of each sex were in each dose group. Animals were 8-9 wks at beginning of exposure.
Time after cessation of dosing
How long the animals were observed after the chemical was no
longer being administered and before death of the animals.
none
Mammary tumors, malignant
Development of malignant mammary gland tumors follows the
same format as for benign, as described above.
0/50, 2/49, 6/49 carcinoma
Comments
This field contains information on the survival rates of the animals and the body
weight trends in order to evaluate whether these factors were likely to have affected the
generation of mammary gland tumors. Mammary gland tumors tend to develop later in an
animal’s life, so studies with lowered survival could mean that animals died before mammary
gland tumors could develop. Decreased weight (perhaps due to toxicity of the chemical) can
decrease the development of tumors. This field may also contain other comments about the
design or outcome of the study.
The exposure duration was originally intended to last 103 wks, but due to excessive mortality was capped at 60 wks (males) or 61 weeks (females).
Other tumors
A list of other tumors that developed in the study that were treatment related.
lymphoma, heart, lung, forestomach, ovary, brain, liver, Zymbal gland
Endocrine related toxicities
This field reports endocrine related toxicities that appeared in the
study such as testicular atrophy.
gonadal atrophy
CPDB TD50 (mg/kg-d)
Data excerpted from the CPDB database that is defined as the "dose-rate in
mg/kg body wt/day which, if administered chronically for the standard lifespan of the species,
will halve the probability of remaining tumorless throughout that period". The CPDB
calculated values for all tumor endpoints listed as well as for total tumors. The range of
mammary gland tumors TD50s is provided, as well as an overall range.
Not calculated by CPDB.